Anti-WRN antibody, Mouse monoclonal
SIGMA/W0393 - clone 195C, purified from hybridoma cell culture
Synonym: Anti-RECQ3; Anti-RECQL2; Anti-RECQL3; Anti-Werner syndrome helicase; Anti-Werner syndrome protein
MDL Number: MFCD02264220
Product Type: Chemical
antibody form | purified immunoglobulin |
antibody product type | primary antibodies |
biological source | mouse |
clone | 195C, monoclonal |
concentration | ~1 mg/mL |
conjugate | unconjugated |
form | buffered aqueous solution |
isotype | IgG1 |
mol wt | antigen ~167 kDa |
Quality Level | 200 |
shipped in | dry ice |
species reactivity | human |
storage temp. | −20°C |
technique(s) | immunohistochemistry: suitable |
immunoprecipitation (IP): suitable | |
indirect ELISA: suitable | |
western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells | |
UniProt accession no. | Q14191 |
Application: | Anti-WRN antibody has been used in • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • immunohistochemistry • western blotting |
Biochem/physiol Actions: | Werner syndrome ATP-dependent helicase (WRN) protein has been demonstrated to possess three known catalytic activities: 3′-5′ helicase, exonuclease and ATPase activities. The C-terminal region of the protein contains a nuclear localization signal (NLS), thus targeting WRN protein to the nucleus, nucleolus and nucleoplasmic foci. The involvement of WRN in multiple DNA metabolic process suggests its function as a tumor suppressor. Mutations in the WRN protein results in Werner syndrome (WS). The epigenetic inactivation of WRN leads to loss of WRN-exonuclease activity, resulting in increased chromosomal instability and hypersensitivity to chemotherapeutic drugs. |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | Anti-WRN antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 195C produced by the fusion of mouse myeloma cells (p3-NS1/Ag4-1) and splenocytes from BALB/c mice immunized with a recombinant fusion protein fragment of human WRN. Werner syndrome ATP-dependent helicase (WRN) is located on chromosome 8 and is 167 kDa. The WRN protein belongs to the RecQ helicase family of DNA helicases. |
Immunogen: | recombinant fusion protein fragment of human WRN (amino acids 1074-1432). |
Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352203 |