Water
SIGMA/W1754 - PCR Reagent
Synonym: H2O
CAS Number: 7732-18-5
Empirical Formula (Hill Notation): H2O
Molecular Weight: 18.02
EC Number: 231-791-2
MDL Number: MFCD00011332
Linear Formula: H2O
Product Type: Chemical
| bp | 100 °C (lit.) |
| density | 1.000 g/mL at 3.98 °C (lit.) |
| foreign activity | DNase, none detected |
| RNase, none detected | |
| form | liquid |
| InChI | 1S/H2O/h1H2 |
| InChI key | XLYOFNOQVPJJNP-UHFFFAOYSA |
| mp | 0 °C (lit.) |
| packaging | vial of 1.5 mL |
| pH | 5-7 |
| Quality Level | 200 ![]() |
| refractive index | n |
| SMILES string | O |
| sterility | sterile-filtered |
| technique(s) | PCR: suitable |
| vapor density | <1 (vs air) |
| vapor pressure | 3 mmHg |
| Application: | Suitable for polymerase chain reaction (PCR) |
| Application: | Water has been used: • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR) |
| General description: | PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination. |
| Other Notes: | Easily compare specifications for Water products with the Water specification table . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| bp | 100 °C (lit.) |
| mp | 0 °C (lit.) |
| Density | 1.000 g/mL at 3.98 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12191602 |

