Williams’ Medium E
SIGMA/W1878 - With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym: Williams’ E medium
Product Type: Chemical
| components | sodium pyruvate: 0.025 g/L phenol red: no glucose: 2.0 g/L (Dextro) NaHCO3: 2.2 g/L L-glutamine: no |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Williams′ Medium E has been used in the culturing of human hepatocellular carcinoma (HCC) cells and hepatic cells. |
| General description: | Williams′ Medium E was developed by Williams and Gunn as a modification to Williams′ Medium D. The medium is formulated to support long-term cell culture of adult liver epithelial cells. It is a modified Minimum Essential Medium (MEM) with altered glucose and amino acid content. |
| Other Notes: | Phenol red has been shown to interfere with the growth of some cells at low or cloning densities. Use this version of William's E when working with stem cells or cells at low densities. |
| Preparation Note: | Supplement with 0.292 g/L |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |