Wiskostatin
SIGMA/W2270
Synonym: 1-
CAS Number: 253449-04-6
Empirical Formula (Hill Notation): C17H18Br2N2O
Molecular Weight: 426.15
MDL Number: MFCD00218393
Linear Formula: C17H18Br2N2O
Product Type: Chemical
| assay | ≥98% (HPLC) |
| color | white to beige |
| form | powder |
| InChI | 1S/C17H18Br2N2O/c1-20(2)9 |
| InChI key | XUBJEDZHBUPBKL-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | CN(C)CC(O)Cn1c2ccc(Br)cc2 |
| solubility | DMSO: 10 mg/mL, clear |
| storage temp. | 2-8°C |
| Application: | Wiskostatin has been used: • as a small-molecule inhibitor of Wiskott-Aldrich syndrome protein (WASP) in human osteosarcoma U2OS cells and mouse-tail fibroblast cell lines • as a neural (N)-WASP inhibitor in cultured neurons and in the human embryonic kidney (HEK293) cells expressing HA-Parkin • as a neural (N)-WASP inhibitor in dorsal root ganglion (DRG) cells to investigate vascular endothelial growth factor (VEGF) effect on the actin related protein 2/3 complex (Arp 2/3) |
| Biochem/physiol Actions: | Wiskostatin is a selective inhibitor of N-WASP, a ubiquitously expressed member of the Wiskott-Aldrich Syndrome protein (WASp) family that regulates actin polymerization. Wiskostatin inhibits actin-dependent cellular functions, including migration, transmembrane transport and phagocytosis. |
| Biochem/physiol Actions: | Wiskostatin mediates the inhibition of cytokinesis. It interacts with WASP especially at the regulatory guanosine-5′-triphosphate (GTP)ase-binding domain (GBD). |
| General description: | Wiskostatin is a dibrominated carbazole. The N-alkylated side chain has a chiral hydroxyl group and a terminal tertiary amine. |
| Packaging: | 5, 25 mg in glass bottle |
| Hazard Codes | T |
| Risk Statements | 25 |
| Safety Statements | 45 |
| RIDADR | UN 2811 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Purity | ≥98% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |