Anti-WSTF (C-Terminal) antibody produced in rabbit
SIGMA/W3516 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-BAZ1B; Anti-Bromdomain Adjacent to Zn Finger Domain 1B; Anti-Williams Syndrome Transcription Factor; Anti-Williams-Beuren Syndrome Chromosome Region 9
MDL Number: MFCD08705535
Product Type: Chemical
Western Blotting JOURNAL CITATION: Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis. By: Dowdle, J. A., Mehta, M., et al. in PLoS Genet, 2013. PubMed ID: 24244200 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003945), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis. By: Dowdle, J. A., Mehta, M., et al. in PLoS Genet, 2013. PubMed ID: 24244200 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003945), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Nuclear foci of WSTF in 3T3 cells stained by Rabbit Anti-WSTF (C-terminal) (Cat. No. W3516).
Immunoblot Mcf7 cell lysate (Lanes 1-5) were separated on SDS-PAGE and blotted with decreasing amounts of Rabbit Anti-WSTF (Cat. No. W3516). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate. Lanes 1: Antibody concentration 2.0 μg/mL 2: Antibody concentration 1.0 μg/mL 3: Antibody concentration 0.5 μg/mL 4: Antibody concentration 1.0 μg/mL + 10 μg/mL non-relevant peptide 5: Antibody concentration 1.0 μg/mL + 10 μg/mL WSTF immunizing peptide
Immunoprecipitation Rabbit Anti-WSTF (C-terminal) (Cat. No. W3516) was used to immunoprecipitate human WSTF from HEK-293T cell lysate. Immunoprecipitate was separated on SDS-PAGE and blotted. Detection antibody: Rabbit Anti-WSTF (N-terminal) (Cat. No. W3641) Lanes 1: 5 μg antibody 2: Negative control — without cell lysate 3: Negative control — without IP antibody 4: Immunoblotting — MCF7 cell extracts was separated on SDS-PAGE and blotted
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 175 kDa |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): 5-10 μg using from lysates of HEK293-T cells |
| indirect immunofluorescence: 10-20 μg/mL using paraformaldehyde-fixed NIH3T3 cells | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using extracts of MCF-7 breast cancer cell line | |
| UniProt accession no. | Q9UIG0  |
| Application: | Anti-WSTF (C-Terminal) antibody produced in rabbit has been used in western blotting and immunofluorescence. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Williams syndrome transcription factor (WSTF) is implicated in transcription, replication, chromatin remodeling and DNA damage response. It acts as a tyrosine protein kinase. Mutations in this gene are associated with Williams-Beuren syndrome. WSTF might function as an activator of gene expression of cytochrome P450 family 21 subfamily A member 2 (CYP21A2) and CYP19A1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Williams syndrome transcription factor (WSTF) belongs to the WAL/BAZ/WAC protein family, encoded by the bromodomain adjacent to zinc finger domain 1B (BAZ1B) gene. It is a non-secretory protein, which consists of a C-terminal bromodomain adjacent to a PHD (plant homeodomain) finger. |
| Immunogen: | synthetic peptide corresponding to amino acids 1467-1483 of human WSTF, conjugated to KLH via an N-terminal added cysteine residue. The corresponding rat and mouse sequence differ from the immunizing sequence by three amino acids. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |