Wee1, active from rat
SIGMA/W4387 - N-terminal GST-tagged, solution
MDL Number: MFCD03459055
Product Type: Chemical
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| application(s) | cell analysis |
| biological source | rat |
| form | solution |
| mol wt | ~110 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q63802  |
| Application: | Wee1, active from rat may be used to study apoptotic signaling. |
| Biochem/physiol Actions: | Protein inhibitor of Cdk2 kinase |
| Biochem/physiol Actions: | Wee1 is a protein tyrosine kinase that phosphorylates Cdc2 on Tyr15 and hence inactivates the CDK1 and CDK2, which facilitates in the negative regulation of entry of cells into mitosis. Increased level of wee1 stimulates Human Immunodeficiency Virus type 1 (HIV-1) Vpr induced cell cycle arrest at G2/M transition followed by apoptosis. |
| Other Notes: | One unit of Wee 1 is defined as the amount of Wee 1 which inhibits 90% of the activity of 10 units of Cdk2 |
| Physical form: | Solution in 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% Glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |