Water
SIGMA/W4502 - Nuclease-Free Water, for Molecular Biology
Synonym: DNase-Free Water; Not DEPC Treated; Nuclease-Free Water; Protease-Free Water; RNase-Free Water
CAS Number: 7732-18-5
Empirical Formula (Hill Notation): H2O
Molecular Weight: 18.02
EC Number: 231-791-2
MDL Number: MFCD00011332
Linear Formula: H2O
Product Type: Chemical
| bp | 100 °C (lit.) |
| density | 1.000 g/mL at 3.98 °C (lit.) |
| foreign activity | DNase, RNase, Protease, free |
| form | liquid |
| InChI | 1S/H2O/h1H2 |
| InChI key | XLYOFNOQVPJJNP-UHFFFAOYSA |
| mp | 0 °C (lit.) |
| pH | 5-7 |
| Quality Level | 200  |
| refractive index | n |
| SMILES string | O |
| sterility | 0.1 μm filtered |
| technique(s) | electrophoresis: suitable |
| PCR: suitable | |
| vapor density | <1 (vs air) |
| vapor pressure | 3 mmHg |
| Application: | Water has been used- • in cDNA synthesis and PCR amplification • for the dilution of rumen DNA sample prior to qPCR assay • in PCR (polymerase chain reaction) and agarose gel electrophoresis • for the elution of RNA during RNA isolation |
| Features and Benefits: | • Nuclease-Free Water • DNase-Free, RNase-Free, Protease- Free • Not DEPC Treated • 0.1 um Filtered • Prepared from Double Distilled and Deionized Water • Suitable for use in Molecular Applications This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC). |
| Other Notes: | Easily compare specifications for Water products with the Water specification table . |
| Preparation Note: | This product is prepared from double distilled and deionized water |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| bp | 100 °C (lit.) |
| mp | 0 °C (lit.) |
| Density | 1.000 g/mL at 3.98 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12191602 |