Monoclonal Anti-WIPI-1 antibody produced in mouse
SIGMA/W4769 - ~1.5 mg/mL, clone WIPI1-4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ATG18; Anti-WIPI-1α; Anti-WIPI49
Product Type: Chemical
Immunoprecipitation 10 μg of Monoclonal Anti-WIPI-1 Clone: WIPI1-4 (Cat. No. W4769) were used to immunoprecipitate WIPI-1 from whole cell extracts. Detection antibody: Rabbit Anti-WIPI-1 antibody (Cat. No. W2519). Lanes 1. G361; 2. L8; 3. 3T3
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-WIPI-1 Clone: WIPI1-4 (Cat. No. W4769). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T cells overexpressing human WIPI-1; 2. G361; 3. NRK; 4. L8; 5. 3T3; 6. C2C12; 7. MBDK
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | WIPI1-4, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~49 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, bovine, rat |
| storage temp. | −20°C |
| technique(s) | western blot: 2-4 μg/mL using whole extract of G361 cells |
| UniProt accession no. | Q5MNZ9  |
| Application: | Monoclonal Anti-WIPI-1 antibody has been used in immunoblotting, immunoprecipitation. |
| Biochem/physiol Actions: | WIPI-1 (WD repeat domain phosphoinositide-interact |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-WIPI-1 (mouse IgG1 isotype) is derived from the hybridoma WIPI1-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human WIPI-1, conjugated to KLH. WIPI-1 (WD repeat domain phosphoinositide-interact |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |