GenomePlex® Complete Whole Genome Amplification (WGA) Kit
SIGMA/WGA2 - Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
Synonym: Whole genome amplification kit; Whole genome amplification kit
Product Type: Chemical
WGA was performed on increasing concentrations of human genomic DNA.
Amplification product can be detected on an agarose gel with as little as 10 pg of input DNA. Optimal performance is found with 1 to 10 ng of starting material. Increasing the amount of input DNA to 100 ng is not recommended.
Lanes
M: DNA Marker
1 and 2. no template
3 and 4. 1 pg DNA
5 and 6. 10 pg DNA
7 and 8. 100 pg DNA
9 and 10. 1 ng DNA
11 and 12. 10 ng DNA
13 and 14. 100 ng DNA
Amplification product can be detected on an agarose gel with as little as 10 pg of input DNA. Optimal performance is found with 1 to 10 ng of starting material. Increasing the amount of input DNA to 100 ng is not recommended.
Lanes
M: DNA Marker
1 and 2. no template
3 and 4. 1 pg DNA
5 and 6. 10 pg DNA
7 and 8. 100 pg DNA
9 and 10. 1 ng DNA
11 and 12. 10 ng DNA
13 and 14. 100 ng DNA
GenomePlex WGA was performed on genomic DNA isolated from HT29 colon carcinoma cells and from a healthy human male. 2.5 μg of WGA product was labeled with Cy3™ or Cy5 dye using the Genomic DNA Labeling Kit PLUS (Agilent). The entire labeled sample was loaded onto an Agilent Human Genome CGH Microarray 105A. Specific activities were between 28 and 43 for all samples, and always within 50% of samples being compared. The dye swaps (A & B) demonstrate that there was no bias in the DNA labeling and the aberrations detected are consistent with the HT-29 karyotype.
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | whole genome amplification: suitable |
| Application: | GenomePlex® Complete Whole Genome Amplification (WGA) Kit has been used in the amplification of DNA. This kit is also suitable for use with downstream applications including: • microarray analysis • SNP analysis • STR analysis • DNA archiving |
| Features and Benefits: | • Higher yield from minimal template: Amplification of nanogram amounts of genomic DNA to microgram yields (around 10 μg) in less than about three hoursrs • Nanograms of samples can be preserved at –20 °C for future use • Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE) • Whole-genome amplification (WGA) DNA polymerase increases the amplification accuracy • Whole-genome representation with no detectable allele bias • Compatible with many downstream applications such as TaqMan® assays, single nucleotide polymorphism (SNP) analysis, comparative genomic hybridization (CGH) analysis |
| General description: | GenomePlex® Complete Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It contains everything required for whole genome amplification including an optimized enzyme, WGA DNA Polymerase. It can be used in several applications and is suitable for use with purified genomic DNA from a variety of sources including blood cards, whole blood, buccal swabs, soil, plant, and serum. It allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. |
| Legal Information: | GenomePlex is a registered trademark of Takara Bio USA, Inc. |
| Legal Information: | TaqMan is a registered trademark of Roche Molecular Systems, Inc. |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Other Notes: | The sequences of the universal primers provided in this kit are considered proprietary. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | Water, Nuclease-Free Water, for Molecular Biology |