GenomePlex® WGA Reamplification Kit
SIGMA/WGA3 - Reamplification of WGA product with minimal bias
Product Type: Chemical
qPCR Effects of Reamplification. Two 10 ng samples of purified human genomic DNA were amplified with WGA2. Each WGA product was then reamplified five successive times with WGA3. qPCR was performed on each sample using primer pairs representative of eight different single copy loci and the average C(t) was determined. Genomic representation was maintained after five successive reamplifications.
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | whole genome amplification: suitable |
| Application: | GenomePlex® WGA Reamplification Kit has been used to generate DNA probes (μBm-probe and μBsm-probe) labeled with digoxigenin-11-dUTP. It has also been used in the reamplification of WGA2-amplified genomic DNA to generate >10 μg gDNA for microarray hybridization. |
| General description: | GenomePlex® WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It allows the user to obtain even higher yields of desired DNA without disturbing the original, limited source of genomic DNA. The genomic DNA can be reamplified up to five cycles with minimal allele dropout producing an even higher yield. It provides the researcher to generate a representative, ~500-fold amplification of genomic DNA. |
| Legal Information: | GenomePlex is a registered trademark of Takara Bio USA, Inc. |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Other Notes: | The sequences of the universal primers provided in this kit are considered proprietary. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .2 mL; Deoxynucleotide Mix, 10 mM |