Monoclonal Anti-ACTB antibody produced in mouse
SIGMA/WH0000060M1 - clone 3G4-F9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-actin, beta
MDL Number: MFCD00164531
Product Type: Chemical
antibody form | purified immunoglobulin |
antibody product type | primary antibodies |
biological source | mouse |
clone | 3G4-F9, monoclonal |
conjugate | unconjugated |
form | buffered aqueous solution |
GenBank® accession no. | BC001301 |
isotype | IgG1κ |
Quality Level | 100 |
shipped in | dry ice |
species reactivity | human, rat, mouse |
storage temp. | −20°C |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
indirect ELISA: suitable | |
western blot: 1-5 μg/mL | |
UniProt accession no. | P60709 |
Application: | Monoclonal Anti-ACTB antibody produced in mouse has been used in western blots and immunohistochemistry. |
Biochem/physiol Actions: | Actin β (ACTB) is an endogenous housekeeping gene. It is extensively used as a reference gene/protein in quantifying expression levels in tumors. Mutation in the gene is associated with the development of Baraitser–Winter cerebrofrontofacial syndrome (BWCFF). Down regulated expression of the gene has been observed in liver, melanoma, renal, colorectal, gastric, pancreatic, esophageal, lung, breast, prostate, ovarian cancers, leukemia and lymphoma. |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
General description: | Actin β (ACTB) is encoded by the gene mapped to human chromosome 7p22.1. ACTB is a ubiquitously expressed cytoskeletal protein. |
General description: | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. (provided by RefSeq) |
Immunogen: | ACTB (AAH01301, 1 a.a. ~ 375 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDDDIAALVVDNGSGMCKAGFAGDD |
Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
Physical form: | Solution in phosphate buffered saline, pH 7.4 |
RIDADR | NONH for all modes of transport |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352203 |