Monoclonal Anti-ACYP2 antibody produced in mouse
SIGMA/WH0000098M6 - clone 2H7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ACYM; Anti-ACYP; Anti-acylphosphatase 2, muscle type
Product Type: Chemical
Western Blotting Western Blot analysis of ACYP2 expression in transfected 293T cell line by ACYP2 monoclonal antibody, clone 2H7. Lanes Lane 1: ACYP2 transfected lysate (11.1 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of ACYP2 over-expressed 293 cell line, cotransfected with ACYP2 Validated Chimera RNAi. Blot probed with ACYP2 monoclonal antibody, clone 2H7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.52 kDa).
ELISA Detection limit for recombinant GST tagged ACYP2 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2H7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_138448   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P14621 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Acylphosphatase can hydrolyze the phosphoenzyme intermediate of different membrane pumps, particularly the Ca2+/Mg2+-ATPase from sarcoplasmic reticulum of skeletal muscle. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase on the basis of their tissue localization. This gene encodes the muscle-type isoform (MT). An increase of the MT isoform is associated with muscle differentiation. (provided by RefSeq) |
| Immunogen: | ACYP2 (NP_612457, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSTAQSLKSVDYEVFGRVQGVCFRM |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |