Monoclonal Anti-PARP1 antibody produced in mouse
SIGMA/WH0000142M1 - clone 3G4, purified immunoglobulin, buffered aqueous solution
Synonym: PARP1 Antibody - Monoclonal Anti-PARP1 antibody produced in mouse; Parp1 Antibody; Anti-ADPRT; Anti-ADPRT1; Anti-PARP; Anti-PARP1; Anti-PPOL; Anti-pADPRT1; Anti-poly (ADP-ribose) polymerase family, member 1
MDL Number: MFCD01322527
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PARP1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting PARP1 monoclonal antibody, clone 3G4 Western Blot analysis of PARP1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged PARP1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC037545   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P09874 |
| Application: | Monoclonal Anti-PARP1 antibody produced in mouse has been used in: • western blotting • indirect immunofluorescence • high-throughput cellular thermal shift assay (CESTA HT) |
| Biochem/physiol Actions: | Poly (ADP-ribose) polymerase 1 (PARP1) protein plays a role in DNA repair by catalyzing the polymerization of adenosine diphosphate (ADP)-ribose units. This protein also plays a role in the early response to DNA damage. Mutations in the PARP1 gene is associated with loss of cell viability. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Poly (ADP-ribose) polymerase 1 (PARP1) is a nuclear protein and belongs to the PARP family. This protein is made up of the N-terminal DNA-binding domain, central auto modification domain and C-terminal catalytic domain. PARP1 protein is located on the nucleoli. The PARP1 gene is located on the human chromosome at 1q42.12. |
| Immunogen: | PARP1 (AAH37545, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAESSDKLYRVEYAKSGRASCKKCS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |