Monoclonal Anti-AFP antibody produced in mouse
SIGMA/WH0000174M1 - clone 1G7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-FETA; Anti-HPAFP; Anti-
MDL Number: MFCD00162111
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to AFP on HepG2 cell. [antibody concentration 30 μg/mL]
Western Blotting AFP monoclonal antibody, clone 1G7 Western Blot analysis of AFP expression in HepG2.
Western Blotting Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody, clone 1G7. Lanes Lane 1: AFP transfected lysate (69 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
Immunoprecipitation Immunoprecipitation of AFP transfected lysate using anti-AFP monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with AFP rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged AFP is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC027881   |
| isotype | IgGκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P02771 |
| Application: | Monoclonal Anti-AFP antibody produced in mouse has been used in immunocytochemistry. |
| Biochem/physiol Actions: | Alpha-fetoprotein encoded by the gene AFP is involved in the regulation of fatty acids in both fetal and proliferating adult liver cells. Its expression is found to be increased in acute liver injuries, indicating active liver regeneration. It has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It serves as a tumor marker for HCC (hepatocellular carcinoma). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. (provided by RefSeq) |
| Immunogen: | AFP (AAH27881, 500 a.a. ~ 609 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence CCTSSYANRRPCFSSLVVDETYVPP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |