Monoclonal Anti-AHR antibody produced in mouse
SIGMA/WH0000196M2 - clone 3B12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-aryl hydrocarbon receptor
MDL Number: MFCD00802740
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to AHR on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody, clone 3B12. Lanes Lane 1: AHR transfected lysate (96.147 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi. Blot probed with AHR monoclonal antibody, clone 3B12. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
Immunoprecipitation Immunoprecipitation of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with AHR rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged AHR is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_001621   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P35869 |
| Application: | Monoclonal Anti-AHR antibody produced in mouse has been used in western blot technique. |
| Biochem/physiol Actions: | Aryl hydrocarbon receptor (Ahr) helps in the inhibitory effects of 2-(4-hydroxy-3-methoxyphe |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Aryl hydrocarbon receptor (Ahr) is a helix-loop-helix transcription factor and a heterodimeric protein. It is encoded by the gene mapped to human chromosome 7p211. The encoded protein is a member of the helix-loop-helix/Per-Arnt |
| General description: | This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. (provided by RefSeq) |
| Immunogen: | AHR (NP_001612, 721 a.a. ~ 820 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGSFEPSPYPTTSSLEDFVTCLQLP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |