Monoclonal Anti-AKT1 antibody produced in mouse
SIGMA/WH0000207M3 - clone 2E11, purified immunoglobulin, buffered aqueous solution
Synonym: MGC99656; PKB; PRKBA; RAC; RACALPHA; v-akt murine thymoma viral oncogene homolog
MDL Number: MFCD03096597
Product Type: Chemical
antibody form | purified immunoglobulin |
antibody product type | primary antibodies |
biological source | mouse |
clone | 2E11, monoclonal |
conjugate | unconjugated |
form | buffered aqueous solution |
GenBank® accession no. | BC000479 ![]() ![]() |
isotype | IgG2aκ |
Quality Level | 100 ![]() |
shipped in | dry ice |
species reactivity | human, rat, mouse |
storage temp. | −20°C |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
immunoprecipitation (IP): suitable | |
indirect ELISA: suitable | |
indirect immunofluorescence: suitable | |
western blot: 1-5 μg/mL | |
UniProt accession no. | P31749 ![]() ![]() |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. ![]() |
General description: | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. (provided by RefSeq) |
Immunogen: | AKT1 (AAH00479.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSDVAIVKEGWLHKRGEYIKTWRPR |
Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
Physical form: | Solution in phosphate buffered saline, pH 7.4 |
RIDADR | NONH for all modes of transport |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352203 |