Monoclonal Anti-ALOX15 antibody produced in mouse
SIGMA/WH0000246M4 - clone 3D8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-arachidonate 15-lipoxygenase
MDL Number: MFCD08064406
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to ALOX15 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 μg/mL]
Western Blotting ALOX15 monoclonal antibody, clone 3D8 Western Blot analysis of ALOX15 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (98.56 kDa).
ELISA Detection limit for recombinant GST tagged ALOX15 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC029032   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P16050 |
| Biochem/physiol Actions: | ALOX15 (arachidonate 15-lipoxygenase) is involved in homeostatic as well as pathological responses. ALOX15 is responsible for the oxidation of unsaturated fatty acids (at the 15 position) to active hydroperoxy and epoxy metabolites. It is also responsible for the conversion of linoleic acid to anti-tumor 13-S-hydroxyoctadecadieno |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The gene arachidonate 15-lipoxygenase (ALOX15) is mapped to human chromosome 17p13. It is mainly present in the epithelial cells in the upper airways, reticulocytes, eosinophils and macrophages. ALOX15 belongs to the dioxygenases family. It is an IL-4 (interleukin 4)/IL-13 target gene. |
| Immunogen: | ALOX15 (AAH29032, 1 a.a. ~ 662 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGLYRIRVSTGASLYAGSNNQVQLW |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |