Monoclonal Anti-ANXA3 antibody produced in mouse
SIGMA/WH0000306M12 - clone 4F1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ANX3; Anti-annexin A3
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of ANXA3 over-expressed 293 cell line, cotransfected with ANXA3 Validated Chimera RNAi. Blot probed with ANXA3 monoclonal antibody, clone 4F1. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody, clone 4F1. Lanes Lane 1: ANXA3 transfected lysate (37.145 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (61.27 kDa).
ELISA Detection limit for recombinant GST tagged ANXA3 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC000871   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P12429 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. (provided by RefSeq) |
| Immunogen: | ANXA3 (AAH00871, 1 a.a. ~ 323 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MASIWVGHRGTVRDYPDFSPSVDAE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |