Monoclonal Anti-ARF5 antibody produced in mouse
SIGMA/WH0000381M1 - clone 1B4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to ARF5 on HeLa cell. [antibody concentration 15 μg/mL]
Western Blotting ARF5 monoclonal antibody, clone 1B4. Western Blot analysis of ARF5 expression in Raw 264.7.
Western Blotting Western Blot analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody, clone 1B4. Lanes Lane 1: ARF5 transfected lysate (20.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting ARF5 monoclonal antibody, clone 1B4. Western Blot analysis of ARF5 expression in PC-12.
Western Blotting ARF5 monoclonal antibody, clone 1B4. Western Blot analysis of ARF5 expression in HeLa.
Western Blotting ARF5 monoclonal antibody, clone 1B4 Western Blot analysis of ARF5 expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged ARF5 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC003043   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P84084 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. (provided by RefSeq) |
| Immunogen: | ARF5 (AAH03043, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence YFQNTQGLIFVVDSNDRERVQESAD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |