Monoclonal Anti-RHOC antibody produced in mouse
SIGMA/WH0000389M1 - clone 2E12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ARH9; Anti-ARHC; Anti-RHOH9; Anti-ras homolog gene family, member C
Product Type: Chemical
Western Blotting RHOC monoclonal antibody, clone 2E12 Western Blot analysis of RHOC expression in Jurkat.
Western Blotting RHOC monoclonal antibody, clone 2E12. Western Blot analysis of RHOC expression in PC-12.
Western Blotting Western Blot analysis of RHOC expression in transfected 293T cell line by RHOC monoclonal antibody, clone 2E12. Lanes Lane 1: RHOC transfected lysate (22 kDa). Lane 2: Non-transfected lysate.
Western Blotting RHOC monoclonal antibody, clone 2E12. Western Blot analysis of RHOC expression in NIH/3T3.
Western Blotting QC Western Blot detection against Immunogen (46.97 kDa).
ELISA Detection limit for recombinant GST tagged RHOC is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC007245   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P08134 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. (provided by RefSeq) |
| Immunogen: | RHOC (AAH07245, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAAIRKKLVIVGDGACGKTCLLIVF |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |