Monoclonal Anti-ASNA1 antibody produced in mouse
SIGMA/WH0000439M3 - clone 2H3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ARSA1; Anti-ARSAI; Anti-MGC3821; Anti-arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting ASNA1 monoclonal antibody, clone 2H3 Western Blot analysis of ASNA1 expression in HeLa.
Western Blotting Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody, clone 2H3. Lanes Lane 1: ASNA1 transfected lysate (38.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting ASNA1 monoclonal antibody, clone 2H3. Western Blot analysis of ASNA1 expression in PC-12.
Enhanced Validation-RNAi Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi. Blot probed with ASNA1 monoclonal antibody, clone 2H3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged ASNA1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2H3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_004317   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43681 |
| Biochem/physiol Actions: | Asna1/TRC40 interacts with the transmembrane domains (TMD) of newly released tail-anchored (TA) proteins and transfers them to the endoplasmic reticulum (ER) receptor for subsequent membrane insertion and thus, plays an essential role in TA protein biogenesis. The encoded protein is necessary for effective HSV1 replication. Asna1 acts as a key regulator of insulin signaling in C. elegans and mammalian cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | ASNA1 is the human homolog of the bacterial arsA gene. In E. coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials. |
| General description: | TMD recognition complex of 40 kDa (TRC40) also known as arsA arsenite transporter, ATP-binding, homolog 1 (ASNA1), is encoded by the gene mapped to human chromosome 19p13.2. ASNA1 is the mammalian ortholog of yeast Arr4/Get3 gene. The encoded protein is abundantly expressed in pancreatic β cells. |
| Immunogen: | ASNA1 (NP_004308, 239 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PEQTTFICVCIAEFLSLYETERLIQ |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |