Monoclonal Anti-ATF4 antibody produced in mouse
SIGMA/WH0000468M1 - clone 2B3, purified immunoglobulin, buffered aqueous solution
Synonym: ATF4 Antibody - Monoclonal Anti-ATF4 antibody produced in mouse; Atf4 Antibody; Anti-CREB2; Anti-TAXREB67; Anti-TXREB; Anti-activating transcription factor 4 (tax-responsive enhancer element B67)
MDL Number: MFCD01092263
Product Type: Chemical
Western Blotting JOURNAL CITATION: Small molecule inhibition of IRE1α kinase/RNase has anti-fibrotic effects in the lung. By: Thamsen, M., Ghosh, R., et al. in PLoS One, 2019. PubMed ID: 30625178 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0209824), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Disrupted autophagy after spinal cord injury is associated with ER stress and neuronal cell death. By: Liu, S., Sarkar, C., et al. in Cell Death Dis, 2015. PubMed ID: 25569099 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/cddis2014527), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Immunofluorescence of monoclonal antibody to ATF4 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of ATF4 expression in transfected 293T cell line by ATF4 monoclonal antibody, clone 2B3. Lanes Lane 1: ATF4 transfected lysate (38.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged ATF4 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_001675   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P18848 |
| Application: | Monoclonal Anti-ATF4 antibody produced in mouse has been used in immunocytochemistry and western blotting. |
| Biochem/physiol Actions: | Activating transcription factor 4 (ATF4) induces cAMP-dependent renal cyst formation. It is implicated in the regulation of genes involved in various cellular processes such as oxidative stress, amino acid synthesis, differentiation, metastasis and angiogenesis. Elevated expression of ATF4 has been observed in cancer patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Activating transcription factor 4 (ATF4), is a stress-induced transcription factor, encoded by the gene mapped to human chromosome 22q13.1. ATF4 is a member of ATF/CREB (cyclic AMP response element binding protein) family of basic region-leucine zipper (bZip) transcription factors. |
| General description: | This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. (provided by RefSeq) |
| Immunogen: | ATF4 (NP_001666.2, 171 a.a. ~ 270 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SSTPDHSFSLELGSEVDITEGDRKP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |