Monoclonal Anti-ATOX1 antibody produced in mouse
SIGMA/WH0000475M1 - clone 2E6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ATX1; Anti-ATX1 antioxidant protein 1 homolog (yeast); Anti-HAH1; Anti-MGC138453; Anti-MGC138455
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to ATOX1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting ATOX1 monoclonal antibody, clone 2E6. Western Blot analysis of ATOX1 expression in human liver.
Western Blotting ATOX1 monoclonal antibody, clone 2E6. Western Blot analysis of ATOX1 expression in COLO 320 HSR.
Western Blotting ATOX1 monoclonal antibody, clone 2E6 Western Blot analysis of ATOX1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (33.22 kDa).
ELISA Detection limit for recombinant GST tagged ATOX1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_004045   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O00244 |
| Application: | Monoclonal Anti-ATOX1 antibody has been used in immunoblot analysis. |
| Biochem/physiol Actions: | ATOX1 (antioxidant 1 copper chaperone) participates in the human copper modulation system. It plays a major role in the migration of breast cancer cells. ATOX1 helps in the transportation of copper to the cell secretory pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome. (provided by RefSeq) ATOX1 (antioxidant 1 copper chaperone) is a metallochaperone which is also called as HAH1. It is a small cytosolic protein. ATOX1 is located on human chromosome 5q33. |
| Immunogen: | ATOX1 (NP_004036, 1 a.a. ~ 68 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MPKHEFSVDMTCGGCAEAVSRVLNK |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |