Monoclonal Anti-BCL2L1 antibody produced in mouse
SIGMA/WH0000598M1 - clone 3E2-2A5, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BCL2-like 1; Anti-BCL2L; Anti-BCLX; Anti-BCLXL/S; Anti-BclX; Anti-DKFZp781P2092; Anti-bclxL; Anti-bclxS
MDL Number: MFCD00675212
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to BCL2L1 on A-431 cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between BID and BCL2L1. HeLa cells were stained with anti-BID rabbit purified polyclonal 1:1200 and anti-BCL2L1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting BCL2L1 monoclonal antibody, clone 3E2-2A5 Western Blot analysis of BCL2L1 expression in A-431.
Western Blotting Western Blot analysis of BCL2L1 expression in transfected 293T cell line by BCL2L1 monoclonal antibody, clone 3E2-2A5. Lanes Lane 1: BCL2L1 transfected lysate (Predicted MW: 26 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (51.37 kDa).
ELISA Detection limit for recombinant GST tagged BCL2L1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E2-2A5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC019307   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q07817 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. (provided by RefSeq) |
| Immunogen: | BCL2L1 (AAH19307, 1 a.a. ~ 233 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSQSNRELVVDFLSYKLSQKGYSWS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |