Monoclonal Anti-BGN antibody produced in mouse
SIGMA/WH0000633M1 - clone 4E1-1G7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DSPG1; Anti-PGI; Anti-PGS1; Anti-SLRR1A; Anti-biglycan
MDL Number: MFCD02097275
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 1 ~ 10 μg/mL]
Western Blotting BGN monoclonal antibody, clone 4E1-1G7 Western Blot analysis of BGN expression in HepG2.
Western Blotting Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody, clone 4E1-1G7. Lanes Lane 1: BGN transfected lysate (41.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (66.22 kDa).
Immunoprecipitation Immunoprecipitation of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with BGN rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged BGN is 0.03 ng/mL as a capture antibody.
ELISA Detection limit for recombinant GST tagged BGN is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E1-1G7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC002416   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P21810 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome. (provided by RefSeq) |
| Immunogen: | BGN (AAH02416.1, 1 a.a. ~ 368 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MWPLWRLVSLLALSQALPFEQRGFW |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |