Monoclonal Anti-PRDM1 antibody produced in mouse
SIGMA/WH0000639M1 - clone 2B10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BLIMP1; Anti-MGC118922; Anti-MGC118923; Anti-MGC118924; Anti-MGC118925; Anti-PR domain containing 1, with ZNF domain; Anti-PRDIBF1
Product Type: Chemical
Western Blotting Western Blot analysis of PRDM1 expression in transfected 293T cell line by PRDM1 monoclonal antibody, clone 2B10. Lanes Lane 1: PRDM1 transfected lysate (88 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of PRDM1 over-expressed 293 cell line, cotransfected with PRDM1 Validated Chimera RNAi. Blot probed with PRDM1 monoclonal antibody, clone 2B10. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting PRDM1 monoclonal antibody, clone 2B10. Western Blot analysis of PRDM1 expression in human tongue.
Western Blotting PRDM1 monoclonal antibody, clone 2B10. Western Blot analysis of PRDM1 expression in human spleen.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
ELISA Detection limit for recombinant GST tagged PRDM1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_001198   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O75626 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. (provided by RefSeq) |
| Immunogen: | PRDM1 (NP_001189, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MKMDMEDADMTLWTEAEFEEKCTYI |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |