Monoclonal Anti-BMPR1B antibody produced in mouse
SIGMA/WH0000658M1 - clone 2F3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ALK6; Anti-bone morphogenetic protein receptor, type IB
Product Type: Chemical
Western Blotting Western Blot analysis of BMPR1B expression in transfected 293T cell line by BMPR1B monoclonal antibody, clone 2F3. Lanes Lane 1: BMPR1B transfected lysate (56.9 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of BMPR1B over-expressed 293 cell line, cotransfected with BMPR1B Validated Chimera RNAi. Blot probed with BMPR1B monoclonal antibody (M01) clone 2F3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (79.53 kDa).
ELISA Detection limit for recombinant GST tagged BMPR1B is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC047773   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O00238 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. (provided by RefSeq) |
| Immunogen: | BMPR1B (AAH47773, 14 a.a. ~ 502 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KKEDGESTAPTPRPKVLRCKCHHHC |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |