Monoclonal Anti-ZFP36L1 antibody produced in mouse
SIGMA/WH0000677M2 - clone 1A3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BRF1; Anti-Berg36; Anti-ERF1; Anti-RNF162B; Anti-TIS11B; Anti-cMG1; Anti-zinc finger protein 36, C3H type-like 1
MDL Number: MFCD03791430
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of ZFP36L1 over-expressed 293 cell line, cotransfected with ZFP36L1 Validated Chimera RNAi. Blot probed with ZFP36L1 monoclonal antibody, clone 1A3. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of ZFP36L1 expression in transfected 293T cell line by ZFP36L1 monoclonal antibody, clone 1A3. Lanes Lane 1: ZFP36L1 transfected lysate (36.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.62 kDa).
ELISA Detection limit for recombinant GST tagged ZFP36L1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_004926   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q07352 |
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| General description: | This gene is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors. (provided by RefSeq) |
| Immunogen: | ZFP36L1 (NP_004917, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTTTLVSATIFDLSEVLCKGNKMLN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |