Monoclonal Anti-CA12 antibody produced in mouse
SIGMA/WH0000771M1 - clone 1D4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CAXII; Anti-FLJ20151; Anti-HsT18816; Anti-carbonic anhydrase XII
Product Type: Chemical
Western Blotting Western Blot analysis of CA12 expression in transfected 293T cell line by CA12 monoclonal antibody, clone 1D4. Lanes Lane 1: CA12 transfected lysate (39.5 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of CA12 over-expressed 293 cell line, cotransfected with CA12 Validated Chimera RNAi. Blot probed with CA12 monoclonal antibody, clone 1D4. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged CA12 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_206925   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43570 |
| Biochem/physiol Actions: | Carbonic anhydrase 12 (CA12) is used as a diagnostic marker for lung cancer and oral squamous cell carcinoma.CA12 catalyses the reversible hydration of carbon dioxide into protons and bicarbonates. CA12 is expressed in diffuse astrocytomas and is a biomarker for prognosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. (provided by RefSeq). Carbonic anhydrase 12 (CA12) is a transmembrane protein, which is associated with neoplastic growth. CA12 is expressed in normal human endometrium, epithelial fluid and HCO3- secretion. CA12 gene is located on human chromosome 15q22.2. |
| Immunogen: | CA12 (NP_996808, 25 a.a. ~ 124 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence APVNGSKWTYFGPDGENSWSKKYPS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |