Monoclonal Anti-CAMK2A antibody produced in mouse

SIGMA/WH0000815M1 - clone 2C4, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CAMKA; Anti-KIAA0968; Anti-calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha

Product Type: Chemical

Immunofluorescence Immunofluorescence of monoclonal antibody to CAMK2A on HeLa cell. [antibody concentration 10 μg/mL]

Western Blotting CAMK2A monoclonal antibody, clone 2C4. Western Blot analysis of CAMK2A expression in human stomach.

Western Blotting CAMK2A monoclonal antibody, clone 2C4. Western Blot analysis of CAMK2A expression in RIN-m5F.

Western Blotting QC Western Blot detection against Immunogen (37.29 kDa).

ELISA Detection limit for recombinant GST tagged CAMK2A is approximately 3 ng/mL as a capture antibody.

antibody form	purified immunoglobulin
antibody product type	primary antibodies
biological source	mouse
clone	2C4, monoclonal
conjugate	unconjugated
form	buffered aqueous solution
GenBank accession no.	BC040457
isotype	IgG2aκ
Quality Level	100
shipped in	dry ice
species reactivity	human
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	indirect ELISA: suitable
	indirect immunofluorescence: suitable
	western blot: 1-5 μg/mL
UniProt accession no.	Q9UQM7

General description:	The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. (provided by RefSeq)
Immunogen:	CAMK2A (AAH40457, 305 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPV
Legal Information:	GenBank is a registered trademark of United States Department of Health and Human Services
Physical form:	Solution in phosphate buffered saline, pH 7.4

RIDADR	NONH for all modes of transport
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203