Monoclonal Anti-CAMK2D antibody produced in mouse
SIGMA/WH0000817M2 - clone 1A8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CAMKD; Anti-MGC44911; Anti-
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to CAMK2D on NIH/3T3 cell. [antibody concentration 10 μg/mL]
Enhanced Validation-RNAi Western blot analysis of CAMK2D over-expressed 293 cell line, cotransfected with CAMK2D Validated Chimera RNAi. Blot probed with CAMK2D monoclonal antibody (M02) clone 1A8. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of CAMK2D expression in transfected 293T cell line by CAMK2D monoclonal antibody, clone 1A8. Lanes Lane 1: CAMK2D transfected lysate (54.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC032784   |
| isotype | IgG2bλ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q6PHZ2 |
| Biochem/physiol Actions: | As a subunit of the calcium/calmodulin dependent protein kinase II complex, calcium/calmodulin dependent protein kinase II complex δ (CAMK2D) modulates the functions of ion channels and Ca2+ handling proteins. It also regulates the phosphorylation and acetylation of histones. CAMK2D has been shown to be overexpressed in the myocardium of diabetic patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene encoding calcium/calmodulin dependent protein kinase IIδ (CAMK2D) is localized on human chromosome 4q26. The encoded protein is a member of serine/threonine protein kinase family and the Ca2+/calmodulin-dependent protein kinase subfamily. |
| General description: | The product of this gene belongs to the serine/threonine protein kinase family and to the Ca2+/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: α, β, γ, and δ. The product of this gene is a δ chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns |
| Immunogen: | CAMK2D (AAH32784, 301 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KGAILTTMLATRNFSAAKSLLKKPD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |