Monoclonal Anti-CASP9 antibody produced in mouse
SIGMA/WH0000842M1 - clone 3B8-4G2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-APAF3; Anti-CASPASE9c; Anti-ICELAP6; Anti-MCH6; Anti-caspase 9, apoptosis-related cysteine peptidase
MDL Number: MFCD01864176
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to CASP9 on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between XIAP and CASP9. HeLa cells were stained with anti-XIAP rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting CASP9 monoclonal antibody, clone 3B8-4G2. Western Blot analysis of CASP9 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (71.5 kDa).
ELISA Detection limit for recombinant GST tagged CASP9 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B8-4G2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC002452   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P55211 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. (provided by RefSeq) |
| Immunogen: | CASP9 (AAH02452.1, 1 a.a. ~ 416 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDEADRRLLRRCRLRLVEELQVDQL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |