Monoclonal Anti-RUNX1 antibody produced in mouse
SIGMA/WH0000861M6 - clone 2C10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-AML1; Anti-AML1EVI1; Anti-AMLCR1; Anti-CBFA2; Anti-EVI1; Anti-PEBP2A2; Anti-PEBP2aB; Anti-runt-related transcription factor 1 (acute myeloid leukemia 1; aml1 oncogene)
MDL Number: MFCD07370485
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 μg/mL]
Western Blotting RUNX1 monoclonal antibody, clone 2C10 Western Blot analysis of RUNX1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (36.85 kDa).
ELISA Detection limit for recombinant GST tagged RUNX1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | NM_001001890   |
| isotype | IgGκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q01196 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | RUNX1 (NP_001001890.1, 210 a.a. ~ 310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RVSPHHPAPTPNPRASLNHSTAFNP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |