Monoclonal Anti-CD5L antibody produced in mouse
SIGMA/WH0000922M1 - clone 1C8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-API6; Anti-CD5 antigen-like (scavenger receptor cysteine rich family); Anti-PRO229; Anti-SPALPHA; Anti-Spalpha
Product Type: Chemical
Western Blotting CD5L monoclonal antibody, clone 1C8. Western Blot analysis of CD5L expression in HL-60.
Western Blotting CD5L monoclonal antibody, clone 1C8. Western Blot analysis of CD5L expression in different cell lines.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
Immunoprecipitation Immunoprecipitation of CD5L transfected lysate using anti-CD5L monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with CD5L rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged CD5L is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC033586   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43866 |
| Biochem/physiol Actions: | CD5 molecule like (CD5L) controls lipid metabolism and regulates T-helper (Th) 17 cell pathogenicity. In addition, it also has an ability to regulate leukocyte migration and inflammatory responses. Overexpression of the protein has been observed in atopic dermatitis (AD) patients. Thus, it can be used as a potential biomarker for AD. |
| General description: | CD5 molecule like (CD5L), also known as apoptosis inhibitor of macrophage (AIM), is encoded by the gene mapped to human chromosome 1q23.1. CD5L is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It is a soluble protein expressed generally by macrophages in lymphoid and inflamed tissues. This protein is characterized with a potential region of O-linked glycosylation in a Pro-Ser-Thr-rich polypeptide, separating SRCR domains 1 and 2. |
| Immunogen: | CD5L (AAH33586, 41 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QKGQWGTVCDDGWDIKDVAVLCREL |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |