Monoclonal Anti-CDC25C antibody produced in mouse
SIGMA/WH0000995M1 - clone 3B11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CDC25; Anti-cell division cycle 25C
MDL Number: MFCD02096040
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6 μg/mL]
Western Blotting Western Blot analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody, clone 3B11. Lanes Lane 1: CDC25C transfected lysate (53.312 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi. Blot probed with CDC25C monoclonal antibody, clone 3B11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting CDC25C monoclonal antibody, clone 3B11 Western Blot analysis of CDC25C expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
Immunoprecipitation Immunoprecipitation of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with CDC25C rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged CDC25C is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC019089   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P30307 |
| General description: | This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. (provided by RefSeq) |
| Immunogen: | CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence FRSNQRKMLNLLLERDTSFTVCPDV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |