Monoclonal Anti-CDK4 antibody produced in mouse
SIGMA/WH0001019M3 - clone 4F11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CMM3; Anti-MGC14458; Anti-PSKJ3; Anti-
MDL Number: MFCD00282839
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody, clone 4F11. Lanes Lane 1: CDK4 transfected lysate (33.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting CDK4 monoclonal antibody, clone 4F11. Western Blot analysis of CDK4 expression in HeLa S3 NE.
Western Blotting CDK4 monoclonal antibody, clone 4F11. Western Blot analysis of CDK4 expression in 293.
Enhanced Validation-RNAi Western blot analysis of CDK4 over-expressed 293 cell line, cotransfected with CDK4 Validated Chimera RNAi. Blot probed with CDK4 monoclonal antibody (M03) clone 4F11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (35.97 kDa).
ELISA Detection limit for recombinant GST tagged CDK4 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC003644   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P11802 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. (provided by RefSeq) |
| Immunogen: | CDK4 (AAH03644, 211 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KPLFCGNSEADQLGKIFDLIGLPPE |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |