Monoclonal Anti-CDX2 antibody produced in mouse
SIGMA/WH0001045M1 - clone 1C7, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CDX3; Anti-caudal type homeo box transcription factor 2
MDL Number: MFCD06201931
Product Type: Chemical
Western Blotting CDX2 monoclonal antibody, clone 1C7 Western Blot analysis of CDX2 expression in COLO 320 HSR.
Western Blotting CDX2 monoclonal antibody, clone 1C7. Western Blot analysis of CDX2 expression in human parotid gland.
Western Blotting QC Western Blot detection against Immunogen (60.17 kDa).
ELISA Detection limit for recombinant GST tagged CDX2 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC014461   |
| isotype | IgGκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q99626 |
| Biochem/physiol Actions: | Caudal-related homoeobox transcription factor 2 (CDX2) is very specific for intestinal mucosa maintenance and homeostasis. Along with β-catenin, CDX2 favors claudin-1 expression regulating tight junctions. This in turn is essential for the epithelial-mesenchymal transition (EMT) in colorectal cancer (CRC). Abnormal expression of CDX2 in the gut is implicated in intestinal-type metaplasias. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Caudal-related homoeobox transcription factor 2 (CDX2) comprises a homeotic DNA-binding domain, transcriptional domain, and a regulatory domain. The CDX2 gene is mapped to human chromosome 13q12.2. |
| General description: | The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM |
| Immunogen: | CDX2 (AAH14461.1, 1 a.a. ~ 313 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MYVSYLLDKDVSMYPSSVRHSGGLN |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |