Monoclonal Anti-CFL1 antibody produced in mouse
SIGMA/WH0001072M4 - clone 1A1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CFL; Anti-cofilin 1 (non-muscle)
MDL Number: MFCD04040757
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CFL1 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 1.5 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to CFL1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting CFL1 monoclonal antibody, clone 1A1 Western Blot analysis of CFL1 expression in HeLa.
Western Blotting CFL1 monoclonal antibody, clone 1A1. Western Blot analysis of CFL1 expression in NIH/3T3.
Western Blotting CFL1 monoclonal antibody, clone 1A1. Western Blot analysis of CFL1 expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (44 kDa).
ELISA Detection limit for recombinant GST tagged CFL1 is approximately 10 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC011005   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P23528 |
| Biochem/physiol Actions: | Cofilin-l (CFL1) plays a vital role in the regulation of actin cytoskeleton reorganization. It is also implicated in cancer cell migration and invasion in several types of tumor tissues. Elevated expression of the gene is associated with the development of esophageal squamous cell carcinoma (ESCC). In addition, abnormal expression of the gene leads to vulvar cancer and thus, it can be considered as a potential therapeutic target for the same. CFL1 facilitates cytokinesis, cell motility and morphogenesis. Activated CFL1 helps in preventing cytoskeletal-associated defects in axonal development stimulated by synucleins (Syns). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. |
| General description: | The cofilin 1 (CFL1) gene with four exons is mapped to human chromosome 11q13.1. The gene codes for an ubiquitously expressed, 18.5kDa phosphoprotein. |
| Immunogen: | CFL1 (AAH11005, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MASGVAVSDGVIKVFNDMKVRKSST |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |