Monoclonal Anti-RCC1 antibody produced in mouse
SIGMA/WH0001104M1 - clone 2F1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CHC1; Anti-RCC1I; Anti-regulator of chromosome condensation 1
MDL Number: MFCD01634139
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to RCC1 on formalin-fixed paraffin-embedded human thyroid nodular goiter. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to RCC1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting RCC1 monoclonal antibody, clone 2F1 Western Blot analysis of RCC1 expression in A-431.
Western Blotting Western Blot analysis of RCC1 expression in transfected 293T cell line by RCC1 monoclonal antibody, clone 2F1. Lanes Lane 1: RCC1 transfected lysate (45 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC007300   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P18754 |
| Biochem/physiol Actions: | Regulator of chromosome condensation 1 (RCC1) interacts with chromatin and contributes to the formation of RanGTP gradients, which is required for nucleo-cytoplasmic transport, mitotic spindle formation and nuclear envelope reassembly following mitosis. It is an important cell cycle regulator. The protein functions as a tumor suppressor in gastric carcinoma. |
| General description: | Regulator of chromosome condensation 1 (RCC1) is a nuclear guanine nucleotide exchange factor for the small GTPase Ran enzyme. The gene is located on human chromosome 1p35.3. It is a 45 kDa protein. |
| Immunogen: | RCC1 (AAH07300, 312 a.a. ~ 421 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence EGKAYSLGRAEYGRLGLGEGAEEKS |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |