Monoclonal Anti-CKS2 antibody produced in mouse
SIGMA/WH0001164M1 - clone 2H5-2C4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CDC28 protein kinase regulatory subunit 2; Anti-CKSHS2
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to CKS2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of CKS2 expression in transfected 293T cell line by CKS2 monoclonal antibody, clone 2H5-2C4. Lanes Lane 1: CKS2 transfected lysate (9.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (34.43 kDa).
ELISA Detection limit for recombinant GST tagged CKS2 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2H5-2C4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC006458   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P33552 |
| General description: | CKS2 protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS2 mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects specialized role for the encoded protein. (provided by RefSeq) |
| Immunogen: | CKS2 (AAH06458, 1 a.a. ~ 79 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAHKQIYYSDKYFDEHYEYRHVMLP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |