Monoclonal Anti-AP2S1 antibody produced in mouse
SIGMA/WH0001175M1 - clone 3E4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-AP17; Anti-AP17DELTA; Anti-CLAPS2; Anti-adaptor-related protein complex 2, sigma 1 subunit
Product Type: Chemical
Western Blotting Western Blot analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody, clone 3E4. Lanes Lane 1: AP2S1 transfected lysate (17 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi. Blot probed with AP2S1 monoclonal antibody, clone 3E4. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (41.36 kDa).
ELISA Detection limit for recombinant GST tagged AP2S1 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC006337   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P53680 |
| General description: | One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts. (provided by RefSeq) |
| Immunogen: | AP2S1 (AAH06337, 1 a.a. ~ 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MIRFILIQNRAGKTRLAKWYMQFDD |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |