Monoclonal Anti-CLIC1 antibody produced in mouse
SIGMA/WH0001192M2 - clone 3F9, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-G6; Anti-NCC27; Anti-chloride intracellular channel 1
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.15 μg/mL]
Western Blotting Western Blot analysis of CLIC1 expression in transfected 293T cell line by CLIC1 monoclonal antibody, clone 3F9. Lanes Lane 1: CLIC1 transfected lysate (26.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting CLIC1 monoclonal antibody, clone 3F9 Western Blot analysis of CLIC1 expression in 293.
Western Blotting QC Western Blot detection against Immunogen (52.25 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC064527   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O00299 |
| General description: | Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. (provided by RefSeq) |
| Immunogen: | CLIC1 (AAH64527.1, 1 a.a. ~ 241 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAEEQPQVELFVKAGSDGAKIGNCP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |