Monoclonal Anti-CLTB antibody produced in mouse
SIGMA/WH0001212M1 - clone 4B12-1E3, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-LCB; Anti-clathrin, light polypeptide (Lcb)
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CLTB on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to CLTB on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of CLTB expression in transfected 293T cell line by CLTB monoclonal antibody, clone 4B12-1E3. Lanes Lane 1: CLTB transfected lysate (23.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting CLTB monoclonal antibody, clone 4B12-1E3 Western Blot analysis of CLTB expression in MCF-7.
Western Blotting QC Western Blot detection against Immunogen (48.95 kDa).
ELISA Detection limit for recombinant GST tagged CLTB is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4B12-1E3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC006457   |
| isotype | IgG2bκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P09497 |
| General description: | Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. (provided by RefSeq) |
| Immunogen: | CLTB (AAH06457, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MADDFGFFSSSESGAPEAAEEDPAA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |