Monoclonal Anti-COL5A1 antibody produced in mouse
SIGMA/WH0001289M1 - clone 2F4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-collagen, type V, alpha 1
Product Type: Chemical
Western Blotting COL5A1 monoclonal antibody, clone 2F4 Western Blot analysis of COL5A1 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (90.53 kDa).
ELISA Detection limit for recombinant GST tagged COL5A1 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC008760   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P20908 |
| Biochem/physiol Actions: | Collagen type Vα1 chain (COL5A1) intercalates with type I collagen and forms collagen fibrils. Mutations in the gene claims to affect mechanical properties of collagenous tissues and lead to musculoskeletal diseases. In addition, polymorphism in the gene is also associated with the development of vascular Ehlers-Danlos syndrome (EDS), which is characterized by fragile, hyperextensible skin and joint laxity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The collagen type V α1 chain (COL5A1) gene is mapped to human chromosome 9q34.3. The gene codes for a minor fibrillar collagen called type V collagen. |
| General description: | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. (provided by RefSeq) |
| Immunogen: | COL5A1 (AAH08760, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGPPGPPGPRGPSGAPGADGPQGPP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |