Monoclonal Anti-CREB1 antibody produced in mouse
SIGMA/WH0001385M1 - clone 3F1-1B2, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CREB; Anti-MGC9284; Anti-cAMP responsive element binding protein 1
MDL Number: MFCD01092316
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CREB1 on formalin-fixed paraffin-embedded human lymph node tissue. [antibody concentration 1 ~ 10 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to CREB1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of CREB1 expression in transfected 293T cell line by CREB1 monoclonal antibody, clone 3F1-1B2. Lanes Lane 1: CREB1 transfected lysate (37 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (63.25 kDa).
ELISA Detection limit for recombinant GST tagged CREB1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F1-1B2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC010636   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P16220 |
| General description: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq) |
| Immunogen: | CREB1 (AAH10636, 1 a.a. ~ 341 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTMESGAENQQSGDAAVTEAENQQM |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |