Monoclonal Anti-CTSK antibody produced in mouse
SIGMA/WH0001513M1 - clone 2F1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CTS02; Anti-CTSO; Anti-CTSO1; Anti-CTSO2; Anti-MGC23107; Anti-PKND; Anti-PYCD; Anti-cathepsin K (pycnodysostosis)
MDL Number: MFCD02097890
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CTSK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 μg/mL]
Western Blotting Western Blot analysis of CTSK expression in transfected 293T cell line by CTSK monoclonal antibody, clone 2F1. Lanes Lane 1: CTSK transfected lysate (37 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.95 kDa).
ELISA Detection limit for recombinant GST tagged CTSK is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | BC016058   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P43235 |
| Biochem/physiol Actions: | Cathepsin K has a role in bone resorption and remodeling. It has a triple helical collagen hydrolase activity. Mutations in the gene encoding this protein have been linked to pycnodysostosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. (provided by RefSeq) |
| Immunogen: | CTSK (AAH16058, 220 a.a. ~ 329 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KCRGYREIPEGNEKALKRAVARVGP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |