Monoclonal Anti-DCX antibody produced in mouse
SIGMA/WH0001641M1 - clone 1G12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DBCN; Anti-DC; Anti-LISX; Anti-SCLH; Anti-XLIS; Anti-doublecortex; lissencephaly, X-linked (doublecortin)
MDL Number: MFCD03454961
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to DCX on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of DCX expression in transfected 293T cell line by DCX monoclonal antibody, clone 1G12. Lanes Lane 1: DCX transfected lysate (41.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (65.34 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC027925   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O43602 |
| Biochem/physiol Actions: | Doublecortin (DCX) encodes a protein which binds to microtubule and is involved in neuronal migration through Ca2+ signalling. Mutations in DCX causes defect in neuronal migration, that results in lissencephaly (LIS) or smooth brain in males, and in females, it leads to subcortical laminar heterotopia (SCLH).LIS/SCLH leads to mental retardation or epilepsy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetyl hydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene. (provided by RefSeq) Doublecortin (DCX) is an essential gene for neurodevelopment and is expressed in neuronal progenitor cells and immature migrating neurons in the foetus. DCX is mapped to human chromosome Xq22.3-q23. |
| Immunogen: | DCX (AAH27925, 1 a.a. ~ 360 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MELDFGHFDERDKTSRNMRGSRMNG |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |