Monoclonal Anti-NQO1 antibody produced in mouse
SIGMA/WH0001728M1 - clone 1E3-A6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DHQU; Anti-DIA4; Anti-DTD; Anti-NAD(P)H dehydrogenase, quinone 1; Anti-NMOR1; Anti-NMORI; Anti-QR1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody, clone 1E3-A6. Lanes Lane 1: NQO1 transfected lysate (30.9 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi. Blot probed with NQO1 monoclonal antibody, clone 1E3-A6. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting NQO1 monoclonal antibody, clone 1E3-A6 Western Blot analysis of NQO1 expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (55.88 kDa).
Immunoprecipitation Immunoprecipitation of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with NQO1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged NQO1 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E3-A6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC007659   |
| isotype | IgG1κ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P15559 |
| Biochem/physiol Actions: | NAD(P)H quinone dehydrogenase 1 (NQO1) uses NADH or NADPH as a reducing cofactor and is characterized by its inhibition by dicoumarol. It is thought to confer protection against natural and exogenous quinones. NQO1 is involved in the conversion of benzene derived quinones to less toxic hydroquinones and participates in benzene-associated hematotoxicity. It is involved in the reduction and eventual activation of few chemotherapeutic drugs and environmental carcinogens, such as heterocyclic amines, nitroaromatic compounds and possibly cigarette smoke particulate. NQO1 is up-regulated in serous ovarian carcinoma and this expression is linked with poor prognosis; hence, this gene has potential as a biomarker for the same. |
| General description: | NAD(P)H quinone dehydrogenase 1 (NQO1) is an obligate two-electron reductase. It is a cytoplasmic, homodimeric protein, with one FAD molecule per monomer. This gene is localized to human chromosome 16q23. It shows a wide range of tissue expression. |
| General description: | This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein′s enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer′s disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. (provided by RefSeq) |
| Immunogen: | NQO1 (AAH07659, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MVGRRALIVLAHSERTSFNYAMKEA |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |