Monoclonal Anti-DLX4 antibody produced in mouse
SIGMA/WH0001748M1 - clone 1F11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-BP1; Anti-DLX7; Anti-DLX8; Anti-DLX9; Anti-distal-less homeobox 4
MDL Number: MFCD04040379
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of DLX4 over-expressed 293 cell line, cotransfected with DLX4 Validated Chimera RNAi. Blot probed with DLX4 monoclonal antibody, clone 1F11. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of DLX4 expression in transfected 293T cell line by DLX4 monoclonal antibody, clone 1F11. Lanes Lane 1: DLX4 transfected lysate (26 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.52 kDa).
Immunoprecipitation Immunoprecipitation of DLX4 transfected lysate using anti-DLX4 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with DLX4 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged DLX4 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank® accession no. | BC016145   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q92988 |
| General description: | Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. Three transcript variants have been described for this gene, however, the full length nature of one variant has not been described. Studies of the two splice variants revealed that one encoded isoform functions as a repressor of the beta-globin gene while the other isoform lacks that function. (provided by RefSeq) |
| Immunogen: | DLX4 (AAH16145, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTSLPCPLPGRDASKAVFPDLAPVP |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |