Monoclonal Anti-DPH2 antibody produced in mouse
SIGMA/WH0001802M2 - clone 5B10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-DPH2 homolog (S. cerevisiae); Anti-DPH2L2
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to DPH2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting DPH2 monoclonal antibody, clone 5B10 Western Blot analysis of DPH2 expression in HeLa.
Western Blotting DPH2 monoclonal antibody, clone 5B10. Western Blot analysis of DPH2 expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (37.95 kDa).
ELISA Detection limit for recombinant GST tagged DPH2 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5B10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| GenBank accession no. | NM_001384   |
| isotype | IgG2aκ |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9BQC3 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | DPH2 (NP_001375, 125 a.a. ~ 235 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence RQRSVALELCVKAFEAQNPDPKAPV |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |